Regulatory
Part:BBa_K2095001:Design
Designed by: Michelle Nilsson Group: iGEM16_Linkoping_Sweden (2016-10-09)
U6 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
According to IDT, the U6 promoter sequence is required for a well functioning sgRNA expression when separating sgRNA and Cas9 from the same coding sequence
Source
Recommended by IDT